Project

Part:BBa_K3113318:Design

Designed by: Alejandro Salinas Illarena   Group: iGEM19_Munich   (2019-10-13)


pCAG_mGag-HiBit-L7Ae


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2491
    Illegal BglII site found at 3019
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2421
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence was optimised to function in Mus musculus cells.


Source

CAG promotor, Gag: HIV coat protein, HiBit: small part of nanoluc luciferase, L7Ae : archeal ribosomal RBP

References